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Ezrin 抗体 (pThr566)

Name: Ezrin 抗体 (pThr566)
SKU: ABIN196943
Availability: OutOfStock

EZR 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

Image no. 1 for anti-Ezrin (EZR) (pThr566) antibody (ABIN196943) (Ezrin 抗体 (pThr566))

1 image

产品编号 ABIN196943

发货至: 中国

Datasheet as PDF

抗原 See all Ezrin (EZR) 抗体

Ezrin (EZR)
抗原表位
28

16

10

9

9

9

6

5

5

4

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr566
适用
140

79

69

9

8

6

6

4

4

3

3

1

1

1

1
人, 小鼠
宿主
130

9

3

2
兔
Compatible Secondaries
克隆类型
124

19
多克隆
标记
90

6

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1
This Ezrin antibody is un-conjugated
应用范围
117

55

26

17

16

13

13

13

11

8

3

2

1

1

1

1

1

1

1

1
Western Blotting (WB)

特异性

Ezrin antibody detects endogenous levels of Ezrin only when phosphorylated at threonine 566.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

免疫原

The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 566 (Y-K-TP-L-R).

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应用备注

Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS ((without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

-20 °C
抗原

Ezrin (EZR)

别名

Ezrin (EZR 产品)

背景

Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

基因ID

7430

NCBI登录号

NP_001104547

UniProt

P15311

途径

Maintenance of Protein Location