Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. ASK1 (phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.
纯化方法
Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatography using non-phosphopeptide.
免疫原
Peptide Sequence around the phosphorylation site of Serine 966 (S-I-Sp-L-P) derived from Human ASK1 .
Suitable for use in Suitable for use Immunohistochemistry on paraffin-embedded sections(1: 50-1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody (in aliquots) at -20 °C.
抗原
ASK1 (MAP3K5)
(Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
Mitogen activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1). ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5