RFP 抗体
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- 抗原 See all RFP 抗体
- RFP (Red Fluorescent Protein (RFP))
- 适用
- Discosoma
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This RFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
- 纯化方法
- Protein A purified
- 过滤
- Sterile filtered
- 免疫原
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Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
ImmunogenType: Fusion protein - 克隆位点
- 8E5-G7
- 亚型
- IgG2a
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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (HRP)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP
anti-Red Fluorescent Protein (RFP) antibody (DyLight 405)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 405
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- 应用备注
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ELISA (1:20000 - 1:50000), WB (1:1000 - 1:3000),
Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. - 限制
- 仅限研究用
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- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
- 注意事项
- avoid freeze thaw cycles
- 储存条件
- 4 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, . Centrifuge product before removing cap. Only dilute immediately prior to use.
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- 抗原
- RFP (Red Fluorescent Protein (RFP))
- 别名
- RFP / Red Fluorescent Protein (RFP 产品)
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