RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis.
纯化方法
Salt fractionation
免疫原
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Usage: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制
仅限研究用
状态
Liquid
溶解方式
Distilled water
浓度
Lot specific
缓冲液
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA
注意事项
avoid freeze thaw cycles
储存条件
4 °C/-20 °C
储存方法
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.