Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA
特异性
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
纯化方法
Protein A purified
过滤
Sterile filtered
免疫原
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.
Usage: Anti-BrdU Antibody has been tested as suitable for immunoblot assays. Antibody may be suitable for additional immunoassays including immunofluorescence, flow cytometry and immunohistochemistry. Antibody will detect incorporated BrdU thymidine analog from replicated cells.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, sterile filtered
注意事项
avoid freeze thaw cycles
储存条件
4 °C,-20 °C
储存方法
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.