(2 references)-
- 抗原 See all MICA 抗体
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MICA antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- PHA-activated peripheral blood lymphocytes
- 克隆位点
- MEM-147
- 亚型
- IgG1
- Top Product
- Discover our top product MICA Primary Antibody
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anti-MHC Class I Polypeptide-Related Sequence A (MICA) (AA 68-97) antibody
MICA 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB21777 unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) (AA 120-170) antibodyMICA 适用: 人 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) (AA 1-200) antibodyMICA 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibodyMICA 适用: 人 WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-147 unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibodyMICA 适用: 人, Cow, Cat, Non-Human Primate ELISA, FACS, IP, ICC, IHC (fro), CyTOF 宿主: 小鼠 Monoclonal W6-32 unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibodyMICA 适用: 人, Cow, Cat, Non-Human Primate ELISA, FACS, IP, ICC, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal W6-32 unconjugated
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibody (FITC)MICA 适用: 人, Cow, Cat, Non-Human Primate FACS 宿主: 小鼠 Monoclonal W6-32 FITC
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibody (Biotin)MICA 适用: 人, Cow, Cat, Non-Human Primate ELISA, FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal W6-32 Biotin
anti-MHC Class I Polypeptide-Related Sequence A (MICA) antibody (PE)MICA 适用: 人, Cow, Cat, Non-Human Primate FACS 宿主: 小鼠 Monoclonal W6-32 PE
anti-MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-307) antibodyMICA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Flow cytometry: Recommended dilution: 1-2 μg/mL.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).
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: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
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- 抗原
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- 别名
- HLA-Class I (MICA 产品)
- 别名
- MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody
- 背景
- HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
- 途径
- Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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