我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD53 抗体 (PE)

Name: CD53 抗体 (PE)
Brand: antibodies-online
SKU: ABIN192139
Availability: OutOfStock

CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD53 (MEM-53) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD53 抗体 (PE))

FACS

Surface staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE. (CD53 抗体 (PE))

FACS

Separation of human monocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD53 (MEM-53) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD53 抗体 (PE))

FACS

3 images

(5 references)

产品编号 ABIN192139

发货至: 中国

Datasheet as PDF

抗原 See all CD53 抗体

CD53
适用
64

4

3
人
宿主
41

23
小鼠
Compatible Secondaries
克隆类型
40

22

2
单克隆
标记
26

5

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD53 antibody is conjugated to PE
应用范围
42

29

25

13

13

12

9

9

8

7

3

2

1

1
Flow Cytometry (FACS)

特异性

The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Leukocytes of pacient suffering from a LGL-type leukemia.

克隆位点

MEM-53

亚型

IgG1

Top Product

Discover our top product CD53 Primary Antibody
anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7A8D3 unconjugated

anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1H2C7 unconjugated

anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated

anti-CD53 (CD53) (AA 100-180) antibody
CD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody

anti-CD53 (CD53) (Internal Region) antibody
CD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD53 (CD53) antibody (FITC)
CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC

anti-CD53 (CD53) (AA 65-160) antibody
CD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-CD53 (CD53) antibody (PE)
CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal 4AC53-2 PE

anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, IF, Func 宿主: 兔 Chimeric 161-2 unconjugated Recombinant Antibody

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Szöllósi, Horejsí, Bene, Angelisová, Damjanovich: "Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2939-46, (1996) (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).

Rasmussen, Blomhoff, Stokke, Horejsi, Smeland: "Cross-linking of CD53 promotes activation of resting human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4997-5007, (1994) (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, (1990) (PubMed).
抗原

CD53

别名

CD53 (CD53 产品)

别名

AI323659 antibody, Ox-44 antibody, Tspan25 antibody, MOX44 antibody, TSPAN25 antibody, OX44 antibody, CD53 antigen antibody, CD53 molecule antibody, Cd53 molecule antibody, Cd53 antibody, CD53 antibody

背景

CD53 Molecule,CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.,Tetraspanin-25, MOX44, TSPAN25

基因ID

963

UniProt

P19397