This antibody detects Bone Sialoprotein. There were no cross reactivities obtained with Human Osteonectin, Human Osteopontin and Human Alkaline Phosphatase.
ELISA (1 μg/mL). RIA (20 ng/mL). Flow Cytometry. Western blot (2 ng/mL). Immunohistochemistry on Paraffin Sections (2 ng/mL). Immunocytochemistry (1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in aqua bidest to initial volume.
缓冲液
PBS, pH 7.4 without preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized at 2-8 °C and reconstituted at -20 °C.
The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12 % of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.Synonyms: BNSP, BSP II, Bone sialoprotein II, Cell-binding sialoprotein, IBSP, Integrin-binding sialoprotein