MAPK6 抗体 (AA 683-694)
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- 抗原 See all MAPK6 抗体
- MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))
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抗原表位
- AA 683-694
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适用
- 人, 小鼠, Cow, 犬, 马, 兔, Pig, 小鸡, Hamster
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This MAPK6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4, NP_081694.1).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Rat, Xenopus (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MAPK6 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (0.5 - 1 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))
- 别名
- MAPK6 / ERK3 (MAPK6 产品)
- 别名
- ERK3 antibody, HsT17250 antibody, PRKM6 antibody, p97MAPK antibody, 2610021I23Rik antibody, D130053K17Rik antibody, Erk3 antibody, Mapk4 antibody, Mapk63 antibody, Prkm4 antibody, Prkm6 antibody, ATMAPK6 antibody, ATMPK6 antibody, F18O19.10 antibody, MAP KINASE 6 antibody, MAP kinase 6 antibody, MAPK6 antibody, SUSERK3 antibody, erk3 antibody, p97mapk antibody, si:ch211-235f12.1 antibody, mitogen-activated protein kinase 6 antibody, MAP kinase 6 antibody, MAPK6 antibody, Mapk6 antibody, MPK6 antibody, mapk6 antibody
- 背景
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Name/Gene ID: MAPK6
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK - 基因ID
- 5597
- NCBI登录号
- NP_002739
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
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