PIWIL1 抗体 (Internal Region)
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- 抗原 See all PIWIL1 抗体
- PIWIL1 (Piwi-Like 1 (PIWIL1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PIWIL1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- PIWI / HIWI
- 序列
- SNRKDKYDAI KKY
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1.
- 亚型
- IgG
- Top Product
- Discover our top product PIWIL1 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PIWIL1 (Piwi-Like 1 (PIWIL1))
- 别名
- PIWIL1 (PIWIL1 产品)
- 别名
- ziwi antibody, PIWI antibody, HIWI antibody, MIWI antibody, piwi like RNA-mediated gene silencing 1 antibody, PIWI-like protein 1 antibody, putative PIWI-like protein 1 antibody, putative argonaut-like protein antibody, piwi-like RNA-mediated gene silencing 1 antibody, PIWIL1 antibody, Tb10.70.5520 antibody, LINJ_21_0470 antibody, LBRM_21_0470 antibody, piwil1 antibody, Piwil1 antibody
- 背景
- PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi, MIWI, piwi homolog, piwi-like 1
- 基因ID
- 9271, 57749
- NCBI登录号
- NP_004755, NP_001177900
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