PIAS2 抗体 (Internal Region)
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- 抗原 See all PIAS2 抗体
- PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PIAS2 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC)
- 原理
- PIAS2
- 序列
- KEAMKVSSQP CTKIE
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.
- 交叉反应
- 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence KEAMKVSSQPCTKIE, from the internal region of the protein sequence according to NP_775298.1, NP_004662.2.
- 亚型
- IgG
- Top Product
- Discover our top product PIAS2 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5 μg/mL.
Western Blot: Preliminary experiments gave an approx 90 kDa band in lysates of cell line A431after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of
Peptide ELISA: antibody detection limit dilution 1:8000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))
- 别名
- PIAS2 (PIAS2 产品)
- 背景
- PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3
- 基因ID
- 9063, 17344
- NCBI登录号
- NP_775298, NP_004662
- 途径
- JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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