OGT 抗体 (Internal Region)
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- 抗原 See all OGT 抗体
- OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
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抗原表位
- Internal Region
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This OGT antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- OGT
- 序列
- YEHPKDLKLS DGR
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1, NP_858059.1.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.
Western Blot: Approx 110 kDa band observed in Rat Pancreas lysates calculated MW of 116 kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recomme
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
- 别名
- OGT (OGT 产品)
- 别名
- BcDNA:GH04245 antibody, CG10392 antibody, Dmel\\CG10392 antibody, OGT antibody, Ogt antibody, P1201 antibody, SXC antibody, Sxc antibody, l(2)02637 antibody, l(2)NC130 antibody, sxc/Ogt antibody, fm81g08 antibody, ogt antibody, wu:fc12b01 antibody, wu:fm81g08 antibody, HRNT1 antibody, O-GLCNAC antibody, 1110038P24Rik antibody, 4831420N21Rik antibody, AI115525 antibody, Ogtl antibody, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antibody, super sex combs antibody, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antibody, O-linked N-acetylglucosamine (GlcNAc) transferase antibody, O-linked GlcNAc transferase antibody, o-linked GlcNAc transferase antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, ogt.L antibody, sxc antibody, ogt.1 antibody, OGT antibody, ogt antibody, GL50803_12081 antibody, GL50803_41701 antibody, THAPSDRAFT_264441 antibody, spyA antibody, Ogt antibody
- 背景
- OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC , O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylgl
- 基因ID
- 8473, 108155, 26295
- NCBI登录号
- NP_858058, NP_858059
- 途径
- Regulation of Carbohydrate Metabolic Process
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