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OGT 抗体 (Internal Region)

Name: OGT 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN190863
Availability: OutOfStock

Verified OGT 适用: 人, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

ABIN190863 (0. (OGT 抗体 (Internal Region))

ABIN190863 (5µg/ml) staining of paraffin embedded Human Cortex. (OGT 抗体 (Internal Region))

IHC

ABIN190863 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (OGT 抗体 (Internal Region))

ABIN190863 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (OGT 抗体 (Internal Region))

ABIN190863 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0. (OGT 抗体 (Internal Region))

FACS

ABIN190863 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. (OGT 抗体 (Internal Region))

6 images

产品编号 ABIN190863

发货至: 中国

Datasheet as PDF

抗原 See all OGT 抗体

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
抗原表位
5

5

4

2

2

1

1

1

1
Internal Region
适用
55

31

27

2

2

2

2

2

2

1

1

1

1

1
人, 大鼠
宿主
54

2
山羊
Compatible Secondaries
克隆类型
42

14
多克隆
标记
31

3

3

3

3

3

3

3

3

1
This OGT antibody is un-conjugated
应用范围
32

15

15

11

10

7

5

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

原理

OGT

序列

YEHPKDLKLS DGR

特异性

This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

交叉反应

Cow, 犬, 人, 小鼠, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1, NP_858059.1.

亚型

IgG
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 857-1036) antibody
OGT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IF, IHC (p), IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 606-1022) antibody
OGT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 8G7 unconjugated Recombinant Antibody

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.
Western Blot: Approx 110 kDa band observed in Rat Pancreas lysates calculated MW of 116 kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the
Peptide ELISA: antibody detection limit dilution 1:64000.

说明

Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recomme

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

别名

OGT (OGT 产品)

背景

OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC , O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylgl

基因ID

8473, 108155, 26295

NCBI登录号

NP_858058, NP_858059

途径

Regulation of Carbohydrate Metabolic Process