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ABHD5 抗体 (Internal Region)

Verified ABHD5 适用: 人, 小鼠 ELISA, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN190850
发货至: 中国
  • 抗原 See all ABHD5 抗体
    ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))
    抗原表位
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 31
    • 9
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    人, 小鼠
    宿主
    • 26
    • 6
    山羊
    克隆类型
    • 27
    • 5
    多克隆
    标记
    • 22
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ABHD5 antibody is un-conjugated
    应用范围
    • 19
    • 13
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    原理
    CGI58 / ABHD5
    序列
    FPERPDLADQ DR
    特异性
    This antibody is not expected to cross-react with ABHD4.
    交叉反应
    犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.
    亚型
    IgG
    Top Product
    Discover our top product ABHD5 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1 kDa according to NP_057090.2). Additional faint bands of 26 kDa and 18 kDa were also observed, and were successfully blocked by incubation with the immunizin

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Koltes, Spurlock: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." in: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).

  • 抗原
    ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))
    别名
    ABHD5 (ABHD5 产品)
    背景
    ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
    基因ID
    51099, 67469, 316122
    NCBI登录号
    NP_057090
    途径
    Lipid Metabolism
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