ASS1 抗体 (Internal Region)
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- 抗原 See all ASS1 抗体
- ASS1 (Argininosuccinate Synthase 1 (ASS1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ASS1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Argininosuccinate synthetase 1
- 序列
- ENPKNQAPPG LYTKTQD
- 特异性
- The variants represent identical protein (NP_000041.2 and NP_446464.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-ENPKNQAPPGLYTKTQD
- 亚型
- IgG
- Top Product
- Discover our top product ASS1 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
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Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
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- 抗原
- ASS1 (Argininosuccinate Synthase 1 (ASS1))
- 别名
- ASS1 (ASS1 产品)
- 别名
- ASS antibody, CTLN1 antibody, AA408052 antibody, Ass-1 antibody, fold antibody, ASSA antibody, Ass antibody, ass antibody, zgc:92051 antibody, wu:fb95a04 antibody, wu:fc01e08 antibody, argininosuccinate synthase 1 antibody, argininosuccinate synthetase 1 antibody, argininosuccinate synthase 1 S homeolog antibody, ASS1 antibody, Ass1 antibody, ass1.S antibody, ass1 antibody
- 背景
- Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
- 基因ID
- 445, 11898, 25698
- NCBI登录号
- NP_000041, NP_446464
- 途径
- Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
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