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ASS1 抗体 (Internal Region)

Verified ASS1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN190834
发货至: 中国
  • 抗原 See all ASS1 抗体
    ASS1 (Argininosuccinate Synthase 1 (ASS1))
    抗原表位
    • 12
    • 8
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 59
    • 15
    • 11
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 49
    • 8
    • 2
    山羊
    克隆类型
    • 50
    • 9
    多克隆
    标记
    • 38
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ASS1 antibody is un-conjugated
    应用范围
    • 46
    • 32
    • 27
    • 18
    • 11
    • 8
    • 5
    • 3
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    Argininosuccinate synthetase 1
    序列
    ENPKNQAPPG LYTKTQD
    特异性
    The variants represent identical protein (NP_000041.2 and NP_446464.1).
    交叉反应
    Cow, 犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    C-ENPKNQAPPGLYTKTQD
    亚型
    IgG
    Top Product
    Discover our top product ASS1 Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).

  • 抗原
    ASS1 (Argininosuccinate Synthase 1 (ASS1))
    别名
    ASS1 (ASS1 产品)
    别名
    ASS antibody, CTLN1 antibody, AA408052 antibody, Ass-1 antibody, fold antibody, ASSA antibody, Ass antibody, ass antibody, zgc:92051 antibody, wu:fb95a04 antibody, wu:fc01e08 antibody, argininosuccinate synthase 1 antibody, argininosuccinate synthetase 1 antibody, argininosuccinate synthase 1 S homeolog antibody, ASS1 antibody, Ass1 antibody, ass1.S antibody, ass1 antibody
    背景
    Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
    基因ID
    445, 11898, 25698
    NCBI登录号
    NP_000041, NP_446464
    途径
    Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
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