FH 抗体 (Internal Region)
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- 抗原 See all FH 抗体
- FH (Fumarate Hydratase (FH))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This FH antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
- 原理
- Fumarase / FH
- 序列
- HPNDHVNKSQ SSND
- 预测反应
- Human, Mouse, Rat, Dog
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.
- 亚型
- IgG
- Top Product
- Discover our top product FH Primary Antibody
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anti-Fumarate Hydratase (FH) (AA 45-188) antibody
FH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) antibodyFH 适用: 人, 大鼠, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Fumarate Hydratase (FH) antibodyKO Validated FH 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E10 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D4 unconjugated
anti-Fumarate Hydratase (FH) (AA 42-185) antibodyFH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) antibodyFH 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.
DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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