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FH 抗体 (Internal Region)

Verified FH 适用: 人 WB, ELISA, IHC, EIA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN190758
发货至: 中国
  • 抗原 See all FH 抗体
    FH (Fumarate Hydratase (FH))
    抗原表位
    • 15
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 74
    • 18
    • 16
    • 8
    • 1
    宿主
    • 65
    • 17
    • 1
    山羊
    克隆类型
    • 66
    • 17
    多克隆
    标记
    • 41
    • 10
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FH antibody is un-conjugated
    应用范围
    • 52
    • 33
    • 22
    • 17
    • 13
    • 13
    • 10
    • 10
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
    原理
    Fumarase / FH
    序列
    HPNDHVNKSQ SSND
    预测反应
    Human, Mouse, Rat, Dog
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.
    亚型
    IgG
    Top Product
    Discover our top product FH Primary Antibody
  • 应用备注

    DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

    DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    FH (Fumarate Hydratase (FH))
    别名
    Fumarate Hydratase (FH 产品)
    别名
    FH antibody, DDBDRAFT_0205237 antibody, DDBDRAFT_0231397 antibody, DDBDRAFT_0231400 antibody, DDB_0205237 antibody, DDB_0231397 antibody, DDB_0231400 antibody, HLRCC antibody, LRCC antibody, MCL antibody, MCUL1 antibody, Fh1 antibody, im:7152785 antibody, ns:zf-e152 antibody, zf-e152 antibody, zgc:66253 antibody, zgc:77498 antibody, Fh antibody, Fh-1 antibody, fumarate hydratase antibody, fumarate hydratase 1 antibody, FH antibody, fumH antibody, Fh antibody, fh antibody, Fh1 antibody
    背景
    Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata
    基因ID
    2271
    NCBI登录号
    NP_000134
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