RPA1 抗体
(2 references)Quick Overview for RPA1 抗体 (ABIN190714)
抗原
See all RPA1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
-
预测反应
- Saccharomyces cerevisiae
-
产品特性
- Expected / apparent Molecular Weight of the Antigene: 70 + 30 + 14 kDa
-
纯化方法
- serum
-
免疫原
- RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa), overexpressed in E.coli and purified by chromatography, no affinity tags were added to any of three subunits
-
-
anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (C-Term) antibodyRPA1 适用: 人 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (Internal Region) antibodyRPA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 小鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-240) antibodyRPA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- 1: 20 000 with standard ECL (WB), chromatin immunoprecipiation
-
说明
-
antibody was also successfully used in ChIP application, presented data are courtesy of M. Pool and Dr. H. van Attikum
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- For reconstitution add 50 μL of sterile water
-
注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
-
储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
-
-
: "RPA coordinates DNA end resection and prevents formation of DNA hairpins." in: Molecular cell, Vol. 50, Issue 4, pp. 589-600, (2013) (PubMed).
: "MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context." in: Nucleic acids research, Vol. 41, Issue 5, pp. 3173-89, (2013) (PubMed).
-
: "RPA coordinates DNA end resection and prevents formation of DNA hairpins." in: Molecular cell, Vol. 50, Issue 4, pp. 589-600, (2013) (PubMed).
-
- RPA1 (Replication Protein A1, 70kDa (RPA1))
-
别名
- RFA
-
背景
- Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody
-
分子量
- 70 + 30 + 14 kDa
-
UniProt
- P22336, P26754
-
途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
-
