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H2AFX 抗体 (C-Term)

Name: H2AFX 抗体 (C-Term)
SKU: ABIN1882250
Availability: OutOfStock

H2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 938CT5-1-1 unconjugated

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). (H2AFX 抗体 (C-Term))

Immunohistochemical analysis of paraffin-embedded H. (H2AFX 抗体 (C-Term))

IHC (p)

Immunohistochemical analysis of paraffin-embedded Human Thymus section using Pink1 am2199b. (H2AFX 抗体 (C-Term))

IHC

IHC (p)

4 images

(3 references)

产品编号 ABIN1882250

发货至: 中国

Datasheet as PDF

抗原 See all H2AFX 抗体

H2AFX (H2A Histone Family, Member X (H2AFX))
抗原表位
33

25

18

11

6

5

5

5

5

4

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 115-143, C-Term
适用
167

82

60

26

26

20

17

12

9

3

2

2

2

2

1
人
宿主
148

19

2
小鼠
Compatible Secondaries
克隆类型
127

42
单克隆
标记
108

8

6

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1
This H2AFX antibody is un-conjugated
应用范围
134

51

47

37

32

27

23

19

16

9

7

4

4

3

2

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein G column, followed by dialysis against PBS.

免疫原

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.

克隆位点

938CT5-1-1

亚型

IgG1
anti-H2A Histone Family, Member X (H2AFX) (pSer140) antibody
H2AFX 适用: 人 WB, ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (ubLys119) antibody
H2AFX 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) antibody
H2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 8C5 unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX 适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (C-Term) antibody
H2AFX 适用: 人 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pTyr143) antibody
H2AFX 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Monoclonal 1C14 unconjugated

anti-H2A Histone Family, Member X (H2AFX) (AA 12-141) antibody
H2AFX 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. IHC: 1:50. IHC-P: 1:25. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." in: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." in: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

Park, Chan, Park, Oettinger, Kwon: "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner." in: Nucleic acids research, Vol. 31, Issue 23, pp. 6819-27, (2003) (PubMed).
抗原

H2AFX (H2A Histone Family, Member X (H2AFX))

别名

H2AFX (H2AFX 产品)

别名

H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody

背景

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

分子量

15145

NCBI登录号

NP_002096

UniProt

P16104

途径

Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus