ATG4B 抗体 (C-Term)
(6 references)-
- 抗原 See all ATG4B 抗体
- ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))
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抗原表位
- AA 358-390, C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATG4B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 预测反应
- M
- 纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 免疫原
- This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.
- 克隆位点
- RB7560
- 亚型
- Ig Fraction
- Top Product
- Discover our top product ATG4B Primary Antibody
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anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-221) antibody
ATG4B 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6C3C5 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 350-400) antibodyATG4B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 16-45), (N-Term) antibodyATG4B 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB7496 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 85-200) antibodyATG4B 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 23-53), (N-Term) antibodyATG4B 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 94-106) antibody (HRP)ATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 62-73) antibody (FITC)ATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 94-106) antibody (FITC)ATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 62-73) antibody (Alkaline Phosphatase (AP))ATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 94-106) antibody (Alkaline Phosphatase (AP))ATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
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- 应用备注
- IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 6 months
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: "A high-throughput FRET-based assay for determination of Atg4 activity." in: Autophagy, Vol. 8, Issue 3, pp. 401-12, (2012) (PubMed).
: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).
: "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates." in: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7327-38, (2011) (PubMed).
: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." in: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, (2010) (PubMed).
: "Properties of human malignant glioma cells in vitro." in: Medical biology, Vol. 56, Issue 4, pp. 184-93, (1978) (PubMed).
: "Determinants for the establishment of permanent tissue culture lines from human gliomas." in: Acta pathologica et microbiologica Scandinavica. Section A, Pathology, Vol. 81, Issue 6, pp. 791-805, (1974) (PubMed).
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: "A high-throughput FRET-based assay for determination of Atg4 activity." in: Autophagy, Vol. 8, Issue 3, pp. 401-12, (2012) (PubMed).
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- 抗原
- ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))
- 别名
- ATG4B (ATG4B 产品)
- 别名
- apg4b antibody, APG4B antibody, AUTL1 antibody, 2510009N07Rik antibody, AW048066 antibody, Apg4b antibody, Atg4bl antibody, Autl1 antibody, Aut2b2 antibody, autophagy related 4B cysteine peptidase antibody, autophagy-related 4b antibody, Peptidase family C54 protein antibody, cysteine protease ATG4B antibody, ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody, autophagy related 4B, cysteine peptidase antibody, autophagy related 4B, cysteine peptidase L homeolog antibody, ATG4B antibody, Atg4b antibody, AT3G59950 antibody, Tsp_08582 antibody, Tsp_11599 antibody, atg4b antibody, atg4b.L antibody
- 背景
- Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
- 分子量
- 44294
- NCBI登录号
- NP_037457, NP_847896
- UniProt
- Q9Y4P1
- 途径
- Autophagy
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