This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This LIG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the N-terminal region of human LIG4.
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背景
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.