This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This HIST1H2BJ/HIST1H2BK/HIST3H2BB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HIST1H2BJ/HIST1H2BK/HIST3H2BB.
Benyamin, McRae, Zhu, Gordon, Henders, Palotie, Peltonen, Martin, Montgomery, Whitfield, Visscher: "Variants in TF and HFE explain approximately 40% of genetic variation in serum-transferrin levels." in: American journal of human genetics, Vol. 84, Issue 1, pp. 60-5, (2009) (PubMed).
Beck, Nielsen, Matthiesen, Jensen, Sehested, Finn, Grauslund, Hansen, Jensen: "Quantitative proteomic analysis of post-translational modifications of human histones." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 7, pp. 1314-25, (2006) (PubMed).
Pavri, Zhu, Li, Trojer, Mandal, Shilatifard, Reinberg: "Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II." in: Cell, Vol. 125, Issue 4, pp. 703-17, (2006) (PubMed).
抗原
HIST1H2BJ,HIST1H2BK,HIST3H2BB
背景
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].