PDK1 抗体 (AA 172-399)
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- 抗原 See all PDK1 抗体
- PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
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抗原表位
- AA 172-399
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against PDK1. It has been selected for its ability to recognize PDK1 in immunohistochemical staining and western blotting.
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) corresdonding to Val172~Pro399 with N-terminal His Tag
- 亚型
- IgG
- Top Product
- Discover our top product PDK1 Primary Antibody
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- 应用备注
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Western blotting: 0.5-2 μg/mL
1:250-1000 Immunohistochemistry: 5-20 μg/mL
1:25-100 Immunocytochemistry: 5-20 μg/mL
1:25-100 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
- 别名
- Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1 产品)
- 别名
- ATP5A antibody, B830012B01 antibody, D530020C15Rik antibody, si:zfos-979f1.3 antibody, 3-phosphoinositide dependent protein kinase-1 antibody, pyruvate dehydrogenase kinase 1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) antibody, pyruvate dehydrogenase kinase, isoenzyme 1 antibody, pyruvate dehydrogenase kinase, isozyme 1 antibody, pyruvate dehydrogenase kinase 1 L homeolog antibody, Pdpk1 antibody, PDK1 antibody, ATP5G2 antibody, Pdk1 antibody, pdk1 antibody, pdk1.L antibody
- 背景
- [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
- 途径
- PI3K-Akt Signaling, RTK signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect
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