ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
特异性
The antibody is a rabbit polyclonal antibody raised against ALB conjugated to hrp. It has been selected for its ability to recognize ALB in immunohistochemical staining and western blotting.
纯化方法
Affinity Chromatography
免疫原
Native Protein ALB. The albumins are a family of globular proteins, the most common of which is serum albumin. The albumin family consists of all proteins that are water-soluble, are moderately soluble in concentrated salt solutions, and experience heat denaturation. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Substances containing albumins, such as egg white, are called albuminoids. Its main function is to regulate the colloidal osmotic pressure of blood.
Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
Supplied as solution form in PBS, pH 7.4, containing 0.02 % NaN3, 50 % glycerol.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
4 °C
储存方法
Store at 4 °C for frequent use. Stored at -20 °C to -80 °C in a manual defrost freezer for one year without detectable loss of activity.