ube3a 抗体 (AA 158-407)
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- 抗原 See all ube3a 抗体
- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
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抗原表位
- AA 158-407
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ube3a antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Ubiquitin Protein Ligase E3A (UBE3A)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against UBE3A. It has been selected for its ability to recognize UBE3A in immunohistochemical staining and western blotting.
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Ubiquitin Protein Ligase E3A (UBE3A) corresdonding to Ser158~Leu407 with N-terminal His Tag
- 亚型
- IgG
- Top Product
- Discover our top product ube3a Primary Antibody
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- 应用备注
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
- 别名
- Ubiquitin Protein Ligase E3A (ube3a 产品)
- 背景
- ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
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