Pkc beta 1 抗体 (AA 342-600)
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- 抗原 See all Pkc beta 1 抗体
- Pkc beta 1 (Protein Kinase C, beta 1 (Pkc beta 1))
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抗原表位
- AA 342-600
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适用
- 兔
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宿主
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豚鼠
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克隆类型
- 多克隆
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标记
- This Pkc beta 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1)
- 序列
- MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDD DDKAMADIGS-FNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKDHAFF
- 特异性
- The antibody is a cavia polyclonal antibody raised against PKCb1. It has been selected for its ability to recognize PKCb1 in immunohistochemical staining and western blotting.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Protein Kinase C Beta 1 (PKCb1) corresdonding to Phe342~Phe600 (Accession # P05772)
- 亚型
- IgG
- Top Product
- Discover our top product Pkc beta 1 Primary Antibody
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- 应用备注
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Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- Pkc beta 1 (Protein Kinase C, beta 1 (Pkc beta 1))
- 别名
- Protein Kinase C Beta 1 (Pkc beta 1 产品)
- 别名
- PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC antibody, PRKCB_tv2 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, PRKCB antibody, Prkcb antibody
- 背景
- PKC-B1, PRKCB, PRKCB1
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