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- 抗原 See all CRAF 抗体
- CRAF (C-Raf Proto-Oncogene Serine/Threonine Protein Kinase (CRAF))
- 抗原表位
- AA 342-619
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CRAF antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against CRAF. It has been selected for its ability to recognize CRAF in immunohistochemical staining and western blotting.
- 纯化方法
- Antigen-specific affinity chromatography
- 免疫原
- CRAF (Trp342-Ser619)
- 亚型
- IgG
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- 应用备注
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- Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- 有效期
- 12 months
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- 抗原
- CRAF (C-Raf Proto-Oncogene Serine/Threonine Protein Kinase (CRAF))
- 别名
- C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) (CRAF 产品)
- 别名
- CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, RAF1 antibody
- 背景
- Alternative Names: RAF1, Raf1, V-raf-1 Murine Leukemia Viral Oncogene Homolog 1, Proto-oncogene c-RAF
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