Lipotropin, beta - (AA 179-267) 抗体
适用: 人
WB, ELISA, IHC (p), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Lipotropin, beta -
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抗原表位
- AA 179-267
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适用
- 人
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宿主
- 兔
-
克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 序列
- MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EL TGQRLREGDG PDGPADDGAG AQADLEHSLL VAAEKKDEGP YRMEHFRWGS PPKDKRYGGF MTSEKSQTPL VTLFKNAIIK NAYKKGE
- 特异性
- It has been selected for its ability to recognize bLPH in immunohistochemical staining and Western blotting.
- 纯化方法
- Affinity Chromatography
- 免疫原
- bLPH (AA 179-267)
- 亚型
- IgG
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- 应用备注
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - 说明
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Content: The quality control contains recombinant bLPH (Glu179~Glu267) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- 有效期
- 12 months
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- 抗原
- Lipotropin, beta -
- 别名
- beta-Lipotropic Hormone (bLPH)
- 别名
- ACTH antibody, CLIP antibody, LPH antibody, MSH antibody, NPP antibody, POC antibody, proopiomelanocortin antibody, POMC antibody
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