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GPX4 抗体 (C-Term)

Verified GPX4 适用: 人 WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185669
发货至: 中国
  • 抗原 See all GPX4 抗体
    GPX4 (Glutathione Peroxidase 4 (GPX4))
    抗原表位
    • 8
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 51
    • 42
    • 37
    • 9
    • 3
    • 2
    • 2
    • 1
    • 1
    宿主
    • 52
    • 4
    山羊
    克隆类型
    • 43
    • 13
    多克隆
    标记
    • 24
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPX4 antibody is un-conjugated
    应用范围
    • 33
    • 17
    • 16
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    原理
    GPX4 (Isoform a and c)
    序列
    EEPLVIEKDL PHY
    特异性
    This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
    交叉反应
    Cow, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2, NP_001034937.1.
    亚型
    IgG
    Top Product
    Discover our top product GPX4 Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration: 0.3 μg/mL.

    Western Blot: Approx 19 kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. This antibody has been successfully used in WB on

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunoprecipitation:This antibody has been successfully used in IP on Human: Schoenmakers E et al. (2010) PMID:21084748.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Papp, Lu, Bolderson, Boucher, Singh, Holmgren, Khanna: "SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).

    Schoenmakers, Agostini, Mitchell, Schoenmakers, Papp, Rajanayagam, Padidela, Ceron-Gutierrez, Doffinger, Prevosto, Luan, Montano, Lu, Castanet, Clemons, Groeneveld, Castets, Karbaschi, Aitken, Dixon et al.: "Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. ..." in: The Journal of clinical investigation, Vol. 120, Issue 12, pp. 4220-35, (2010) (PubMed).

  • 抗原
    GPX4 (Glutathione Peroxidase 4 (GPX4))
    别名
    GPX4 (GPX4 产品)
    别名
    GPx-4 antibody, GSHPx-4 antibody, MCSP antibody, PHGPx antibody, snGPx antibody, snPHGPx antibody, Phgpx antibody, gpx-4 antibody, snGpx antibody, cb563 antibody, cb691 antibody, PHGPxa antibody, sb:cb563 antibody, zgc:92253 antibody, wu:fb82e03 antibody, 1700027O09Rik antibody, mtPHGPx antibody, ATGPX4 antibody, F11L15.5 antibody, glutathione peroxidase 4 antibody, GP-GPx4 antibody, glutathione peroxidase 4 antibody, glutathione peroxidase 4a antibody, GPX4 antibody, Gpx4 antibody, gpx4a antibody
    背景
    GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase
    基因ID
    2879
    NCBI登录号
    NP_002076, NP_001034937
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