GPX4 抗体 (C-Term)
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- 抗原 See all GPX4 抗体
- GPX4 (Glutathione Peroxidase 4 (GPX4))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GPX4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- 原理
- GPX4 (Isoform a and c)
- 序列
- EEPLVIEKDL PHY
- 特异性
- This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
- 交叉反应
- Cow, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2, NP_001034937.1.
- 亚型
- IgG
- Top Product
- Discover our top product GPX4 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration: 0.3 μg/mL.
Western Blot: Approx 19 kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. This antibody has been successfully used in WB on
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunoprecipitation:This antibody has been successfully used in IP on Human: Schoenmakers E et al. (2010) PMID:21084748.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).
: "Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. ..." in: The Journal of clinical investigation, Vol. 120, Issue 12, pp. 4220-35, (2010) (PubMed).
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SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).
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- 抗原
- GPX4 (Glutathione Peroxidase 4 (GPX4))
- 别名
- GPX4 (GPX4 产品)
- 别名
- GPx-4 antibody, GSHPx-4 antibody, MCSP antibody, PHGPx antibody, snGPx antibody, snPHGPx antibody, Phgpx antibody, gpx-4 antibody, snGpx antibody, cb563 antibody, cb691 antibody, PHGPxa antibody, sb:cb563 antibody, zgc:92253 antibody, wu:fb82e03 antibody, 1700027O09Rik antibody, mtPHGPx antibody, ATGPX4 antibody, F11L15.5 antibody, glutathione peroxidase 4 antibody, GP-GPx4 antibody, glutathione peroxidase 4 antibody, glutathione peroxidase 4a antibody, GPX4 antibody, Gpx4 antibody, gpx4a antibody
- 背景
- GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase
- 基因ID
- 2879
- NCBI登录号
- NP_002076, NP_001034937
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