Patched 1 抗体 (Internal Region)
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- 抗原 See all Patched 1 (PTCH1) 抗体
- Patched 1 (PTCH1)
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Patched 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 原理
- PTCH (Internal)
- 序列
- HPESRHHPPS NPRQQ
- 特异性
- This antibody is expected to recognise all four reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1). Reported variants represent identical protein (NP_001077075.1, NP_001077076.1, NP_001077073.1, NP_001077074.1).
- 交叉反应
- Cow, 犬, 人, Pig
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.
- 亚型
- IgG
- Top Product
- Discover our top product PTCH1 Primary Antibody
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- 应用备注
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Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).
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Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).
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- 抗原
- Patched 1 (PTCH1)
- 别名
- PTCH1 (PTCH1 产品)
- 别名
- BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody
- 背景
- PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog
- 基因ID
- 5727
- NCBI登录号
- NP_000255, NP_001077072, NP_001077071, NP_001077075
- 途径
- Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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