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Patched 1 抗体 (Internal Region)

Verified PTCH1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185667
发货至: 中国
  • 抗原 See all Patched 1 (PTCH1) 抗体
    Patched 1 (PTCH1)
    抗原表位
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 38
    • 26
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    人, 小鼠
    宿主
    • 35
    • 7
    • 3
    山羊
    克隆类型
    • 39
    • 6
    多克隆
    标记
    • 33
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Patched 1 antibody is un-conjugated
    应用范围
    • 31
    • 22
    • 17
    • 11
    • 6
    • 5
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    原理
    PTCH (Internal)
    序列
    HPESRHHPPS NPRQQ
    特异性
    This antibody is expected to recognise all four reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1). Reported variants represent identical protein (NP_001077075.1, NP_001077076.1, NP_001077073.1, NP_001077074.1).
    交叉反应
    Cow, 犬, 人, Pig
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.
    亚型
    IgG
    Top Product
    Discover our top product PTCH1 Primary Antibody
  • 应用备注

    Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

  • 抗原
    Patched 1 (PTCH1)
    别名
    PTCH1 (PTCH1 产品)
    别名
    BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody
    背景
    PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog
    基因ID
    5727
    NCBI登录号
    NP_000255, NP_001077072, NP_001077071, NP_001077075
    途径
    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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