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Hsc70 抗体 (Internal Region)

Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185659
发货至: 中国
  • 抗原 See all Hsc70 (HSPA8) 抗体
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    抗原表位
    • 14
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 83
    • 48
    • 43
    • 21
    • 17
    • 14
    • 13
    • 11
    • 11
    • 11
    • 10
    • 10
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 72
    • 28
    • 7
    • 1
    山羊
    克隆类型
    • 68
    • 39
    多克隆
    标记
    • 60
    • 19
    • 11
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Hsc70 antibody is un-conjugated
    应用范围
    • 94
    • 50
    • 40
    • 30
    • 29
    • 28
    • 16
    • 14
    • 11
    • 11
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
    原理
    HSPA8 (Isoform 1)
    序列
    EKLQGKINDE DKQK
    特异性
    This antibody is expected to recognise isoform 1 (NP_006588.1) only.
    交叉反应
    犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
    亚型
    IgG
    Top Product
    Discover our top product HSPA8 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).

  • 抗原
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    别名
    HSPA8 (HSPA8 产品)
    背景
    HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh
    基因ID
    3312, 15481, 24468
    NCBI登录号
    NP_006588
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