Hsc70 抗体 (Internal Region)
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- 抗原 See all Hsc70 (HSPA8) 抗体
- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Hsc70 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
- 原理
- HSPA8 (Isoform 1)
- 序列
- EKLQGKINDE DKQK
- 特异性
- This antibody is expected to recognise isoform 1 (NP_006588.1) only.
- 交叉反应
- 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
- 亚型
- IgG
- Top Product
- Discover our top product HSPA8 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:4000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
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Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
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- 抗原
- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
- 别名
- HSPA8 (HSPA8 产品)
- 别名
- hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody
- 背景
- HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh
- 基因ID
- 3312, 15481, 24468
- NCBI登录号
- NP_006588
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