GLI2 抗体 (Internal Region)
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- 抗原 See all GLI2 抗体
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GLI2 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC)
- 原理
- GLI2
- 序列
- DLKEDLDRDD CKQE
- 特异性
- This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
- 预测反应
- Human, Mouse
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.
- 亚型
- IgG
- Top Product
- Discover our top product GLI2 Primary Antibody
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- 应用备注
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DS WB Results: Preliminary experiments gave an approx 55 kDa band in Human Bone Marrow and Ovary lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:2000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
- 别名
- GLI2 (GLI2 产品)
- 别名
- HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody
- 背景
- GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2
- 基因ID
- 2736
- NCBI登录号
- NP_005261
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis
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