ERG 抗体 (C-Term)
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- 抗原 See all ERG 抗体
- ERG (ERG, ETS transcription factor (ERG))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ERG antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- ERG
- 序列
- PNTRLPTSHM PSH
- 特异性
- This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.
- 亚型
- IgG
- Top Product
- Discover our top product ERG Primary Antibody
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- 应用备注
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Western Blot: Approx 55 kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8 kDa according to NP_891548.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- ERG (ERG, ETS transcription factor (ERG))
- 别名
- ERG (ERG 产品)
- 别名
- erg-3 antibody, p55 antibody, erg antibody, erg_A antibody, zgc:103429 antibody, erg_E antibody, ERG antibody, D030036I24Rik antibody, KCNH2 antibody, ERG, ETS transcription factor antibody, v-ets avian erythroblastosis virus E26 oncogene homolog L homeolog antibody, v-ets avian erythroblastosis virus E26 oncogene homolog antibody, v-ets avian erythroblastosis virus E26 oncogene homolog S homeolog antibody, ETS transcription factor antibody, potassium voltage-gated channel subfamily H member 6 antibody, ERG antibody, Erg antibody, erg.L antibody, erg antibody, erg.S antibody, KCNH6 antibody
- 背景
- ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets er
- 基因ID
- 2078, 13876, 170909
- NCBI登录号
- NP_891548, NP_004440, NP_001129626, NP_001129627
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