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PDE4D 抗体 (C-Term)

Name: PDE4D 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN185530
Availability: OutOfStock

Verified PDE4D 适用: 人 WB, ELISA, IHC, EIA 宿主: 山羊 Polyclonal unconjugated

ABIN185530 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. (PDE4D 抗体 (C-Term))

IHC

ABIN185530 staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). (PDE4D 抗体 (C-Term))

ABIN185530 staining (0. (PDE4D 抗体 (C-Term))

ABIN185530 (5µg/ml) staining of paraffin embedded Human Heart. (PDE4D 抗体 (C-Term))

IHC

4 images

(1 reference)

产品编号 ABIN185530

发货至: 中国

Datasheet as PDF

抗原 See all PDE4D 抗体

PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))
抗原表位
16

7

4

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
52

43

42

6

4

3

3

3

3

2

2

1

1
人
宿主
55

1

1
山羊
Compatible Secondaries
克隆类型
55

2
多克隆
标记
29

5

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PDE4D antibody is un-conjugated
应用范围
47

21

18

17

11

10

10

6

5

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

原理

PDE4D

序列

QPEACVIDDR SPDT

特异性

This antiobody is expected to recognize all reported isoforms.

交叉反应

人

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

亚型

IgG
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 164-213) antibody
PDE4D 适用: 人, 大鼠, 小鼠, 马, 小鸡, Pig, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 249-518) antibody
PDE4D 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (C-Term) antibody
PDE4D 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 466-709) antibody
PDE4D 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibody
PDE4D 适用: 人, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (C-Term) antibody
PDE4D 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibody
PDE4D 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IP, IF, ICC, CM 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 401-550) antibody
PDE4D 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 581-809) antibody
PDE4D 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibody
PDE4D 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 4D4 unconjugated Recombinant Antibody

应用备注

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated
Peptide ELISA: antibody detection limit dilution 1:128000.

说明

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Bazhin, Kahnert, Kimpfler, Schadendorf, Umansky: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).
抗原

PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

别名

PDE4D (PDE4D 产品)

别名

ACRDYS2 antibody, DPDE3 antibody, HSPDE4D antibody, PDE43 antibody, PDE4DN2 antibody, STRK1 antibody, CMYA2 antibody, MMGL antibody, 9630011N22Rik antibody, Dpde3 antibody, 4732458A06Rik antibody, 9430063L05Rik antibody, C87016 antibody, D130016K21Rik antibody, D3Bwg1078e antibody, Usmg4 antibody, mKIAA0454 antibody, si:dkey-190g11.2 antibody, phosphodiesterase 4D antibody, phosphodiesterase 4D interacting protein antibody, phosphodiesterase 4D, cAMP specific antibody, phosphodiesterase 4D, cAMP-specific antibody, phosphodiesterase 4D interacting protein (myomegalin) antibody, PDE4D antibody, PDE4DIP antibody, pde4d antibody, Pde4dip antibody, Pde4d antibody

背景

PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie

基因ID

5144

NCBI登录号

NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371

途径

Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling