FZD8 抗体 (C-Term)
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- 抗原 See all FZD8 抗体
- FZD8 (Frizzled Family Receptor 8 (FZD8))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This FZD8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)
- 原理
- FZD8 / frizzled 8
- 序列
- SYPKQMPLSQ V
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.
- 亚型
- IgG
- Top Product
- Discover our top product FZD8 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 3 μg/mL.
Western Blot: Approx 75 kDa band observed in Human Pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B e
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.
Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining):
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- FZD8 (Frizzled Family Receptor 8 (FZD8))
- 别名
- FZD8 (FZD8 产品)
- 别名
- FZ-8 antibody, hFZ8 antibody, FZD8 antibody, Fz-8 antibody, frizzled8 antibody, xfz8 antibody, Xfz8 antibody, frizzled-8 antibody, hfz8 antibody, Fz8 antibody, fzd8 antibody, fz5 antibody, fz8a antibody, fzc antibody, wu:fd02c05 antibody, zg05 antibody, ZG06 antibody, Zfz8b antibody, fz6 antibody, fz8b antibody, fzA antibody, ik:tdsubc_1e1 antibody, xx:tdsubc_1e1 antibody, zfzA antibody, frizzled class receptor 8 antibody, frizzled class receptor 8 S homeolog antibody, frizzled class receptor 8 L homeolog antibody, frizzled class receptor 8a antibody, frizzled class receptor 8b antibody, FZD8 antibody, fzd8.S antibody, fzd8 antibody, Fzd8 antibody, fzd8.L antibody, fzd8a antibody, fzd8b antibody
- 背景
- FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046, FZ-8, hFZ8 , frizzled (Drosophila) homolog 8, frizzled 8
- 基因ID
- 8325, 14370
- NCBI登录号
- NP_114072
- 途径
- WNT signaling
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