KPNB1 抗体 (C-Term)
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- 抗原 See all KPNB1 抗体
- KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
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抗原表位
- C-Term
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This KPNB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Karyopherin (importin) beta 1
- 序列
- RRSKTNKAKT LAT
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 90-95- kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2 kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incu
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
- 别名
- KPNB1 (KPNB1 产品)
- 别名
- Afu1g15720 antibody, AO090005001133 antibody, imb1 antibody, impnb antibody, ipo1 antibody, ipob antibody, ntf97 antibody, IMB1 antibody, IPO1 antibody, IPOB antibody, Impnb antibody, NTF97 antibody, AA409963 antibody, importin beta-1 subunit antibody, Importin beta-1 subunit antibody, karyopherin (importin) beta 1 L homeolog antibody, importin beta 1 antibody, karyopherin subunit beta 1 antibody, karyopherin (importin) beta 1 antibody, AFUA_1G15720 antibody, AOR_1_1974174 antibody, Bm1_57540 antibody, PMAA_065830 antibody, AFLA_083750 antibody, TSTA_085870 antibody, BDBG_07509 antibody, MCYG_00039 antibody, kpnb1.L antibody, LOC100533387 antibody, KPNB1 antibody, Kpnb1 antibody
- 背景
- KPNB1, karyopherin (importin) beta 1, HGNC:6400, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1
- 基因ID
- 3837, 16211, 24917
- NCBI登录号
- NP_002256
- 途径
- Protein targeting to Nucleus
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