CBX5 抗体 (Internal Region)
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- 抗原 See all CBX5 抗体
- CBX5 (Chromobox Homolog 5 (CBX5))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This CBX5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- CBX5 / HP1-Alpha
- 序列
- NKRKSNFSNS ADDIK
- 特异性
- Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.
- 交叉反应
- 犬, 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1, NP_001120793.1, NP_001120794.1.
- 亚型
- IgG
- Top Product
- Discover our top product CBX5 Primary Antibody
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- 应用备注
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Western Blot: Approx 24-25 kDa band observed in lysates of cell lines A43N and K562 (calculated MW of 22.2 kDa according to Human NP_036249.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- CBX5 (Chromobox Homolog 5 (CBX5))
- 别名
- CBX5 (CBX5 产品)
- 别名
- HP1 antibody, HP1A antibody, id:ibd2026 antibody, wu:fc63e02 antibody, wu:fe47g12 antibody, wu:fi03h05 antibody, zgc:158601 antibody, CBX5 antibody, cbx5 antibody, MGC64354 antibody, 2610029O15Rik antibody, C75991 antibody, Hp1a antibody, Hp1alpha antibody, chromobox 5 antibody, chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody, chromobox 5 S homeolog antibody, CBX5 antibody, cbx5 antibody, Cbx5 antibody, cbx5.S antibody
- 背景
- CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha , chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein
- 基因ID
- 23468
- NCBI登录号
- NP_036249, NP_001120793, NP_001120794
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