DARC 抗体 (N-Term)
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- 抗原 See all DARC 抗体
- DARC (Duffy Blood Group, Chemokine Receptor (DARC))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DARC antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
- 原理
- Duffy / FY / DARC
- 序列
- HRAELSPSTE NSSQLDFED
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1, NP_002027.2.
- 亚型
- IgG
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anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-336) antibody
DARC 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 71-330) antibodyDARC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-63) antibodyDARC 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-30), (N-Term) antibodyDARC 适用: 人 WB 宿主: 兔 Polyclonal RB42735 unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) antibodyDARC 适用: 人 ELISA, FACS, BP 宿主: 兔 Chimeric 2C3 unconjugated Recombinant Antibody
anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-336) antibodyDARC 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-50) antibodyDARC 适用: 人 ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Duffy Blood Group, Chemokine Receptor (DARC) (N-Term) antibody (Biotin)Verified DARC 适用: 人 WB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal Biotin
anti-Duffy Blood Group, Chemokine Receptor (DARC) antibody (PE)DARC 适用: 人 IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal PE
anti-Duffy Blood Group, Chemokine Receptor (DARC) (C-Term) antibodyDARC 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3
Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- DARC (Duffy Blood Group, Chemokine Receptor (DARC))
- 别名
- ACKR1 (DARC 产品)
- 背景
- FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294
- 基因ID
- 2532
- NCBI登录号
- NP_001116423, NP_002027
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