HRH2 抗体 (C-Term)
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- 抗原 See all HRH2 抗体
- HRH2 (Histamine Receptor H2 (HRH2))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This HRH2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Histamine Receptor H2
- 序列
- QEEKPLKLQV WSGTE
- 交叉反应
- 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1, NP_001124527.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 50 kDa band observed in Human Tonsil lysates (calculated MW of 46.9 kDa according to Human NP_001354640.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors." in: Neuroscience letters, Vol. 541, pp. 87-92, (2013) (PubMed).
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Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors." in: Neuroscience letters, Vol. 541, pp. 87-92, (2013) (PubMed).
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- 抗原
- HRH2 (Histamine Receptor H2 (HRH2))
- 别名
- HRH2 (HRH2 产品)
- 别名
- H2R antibody, HH2R antibody, HRH2 antibody, histamine receptor H2 antibody, histamine receptor H2a antibody, histamine receptor H 2 antibody, HRH2 antibody, Hrh2 antibody, hrh2a antibody
- 背景
- HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242
- 基因ID
- 3274
- NCBI登录号
- NP_001124527, NP_001354640
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