Nanog 抗体 (Internal Region)
(10 references)-
- 抗原 See all Nanog (NANOG) 抗体
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Nanog antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
- 原理
- NANOG
- 序列
- QNQRMKSKRW QKNN
- 交叉反应
- 犬, 人, Pig
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
- 亚型
- IgG
- Top Product
- Discover our top product NANOG Primary Antibody
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anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 101-200) antibodyNANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-305) antibodyNANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 71-170) antibodyNANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 15-49) antibodyNANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-100) antibodyNANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 20-166) antibodyNANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated
anti-Nanog Homeobox (NANOG) (AA 267-292) antibodyNANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22,7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012,21(
Western Blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells." in: Nature communications, Vol. 7, pp. 10743, (2016) (PubMed).
: "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1246-54, (2014) (PubMed).
: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).
: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).
: "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1." in: Cell transplantation, Vol. 21, Issue 5, pp. 815-25, (2012) (PubMed).
: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).
: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).
: "Complete meiosis from human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 8, pp. 1186-95, (2011) (PubMed).
: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).
: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).
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: "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells." in: Nature communications, Vol. 7, pp. 10743, (2016) (PubMed).
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- 抗原
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- 别名
- NANOG (NANOG 产品)
- 别名
- 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
- 背景
- NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
- 基因ID
- 79923, 71950, 414065
- NCBI登录号
- NP_079141
- 途径
- Stem Cell Maintenance
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