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HMGA1 抗体 (Internal Region)

Verified HMGA1 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185443
发货至: 中国
  • 抗原 See all HMGA1 抗体
    HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
    抗原表位
    • 15
    • 9
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 50
    • 36
    • 22
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 51
    • 9
    • 2
    • 1
    山羊
    克隆类型
    • 55
    • 8
    多克隆
    标记
    • 32
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HMGA1 antibody is un-conjugated
    应用范围
    • 56
    • 17
    • 13
    • 13
    • 12
    • 11
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA
    原理
    HMGA1
    序列
    LASKQEKDGT EK
    特异性
    This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).
    交叉反应
    Cow, 犬, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.
    亚型
    IgG
    Top Product
    Discover our top product HMGA1 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4 μg/mL.

    Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Testis and Ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Additional validation: This antibody has been successfully used in qRT-PCR on Human: Cello et al. (2013) PMID: 23545254.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

  • 抗原
    HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
    别名
    HMGA1 (HMGA1 产品)
    背景
    HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A,
    基因ID
    3159
    NCBI登录号
    NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910
    途径
    Nuclear Hormone Receptor Binding, Autophagy
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