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Arrestin 3 抗体 (Internal Region)

Name: Arrestin 3 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN185395
Availability: OutOfStock

Verified ARRB2 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

ABIN185395 (0. (Arrestin 3 抗体 (Internal Region))

(ABIN185395) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0. (Arrestin 3 抗体 (Internal Region))

FACS

ABIN185395 (4µg/ml) staining of paraffin embedded Human Cerebellum. (Arrestin 3 抗体 (Internal Region))

IHC

(ABIN185395) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (Arrestin 3 抗体 (Internal Region))

(ABIN185395) (1 μg/mL) staining of A549 (A),and U251 (B) cell lysate (35 μg protein in RIPA buffer). (Arrestin 3 抗体 (Internal Region))

7 images

(2 references)

产品编号 ABIN185395

发货至: 中国

Datasheet as PDF

抗原 See all Arrestin 3 (ARRB2) 抗体

Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))
抗原表位
15

6

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Internal Region
适用
52

31

30

6

5

4

4

4

3

2

1

1
人
宿主
45

5

2
山羊
Compatible Secondaries
克隆类型
47

5
多克隆
标记
27

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Arrestin 3 antibody is un-conjugated
应用范围
39

18

15

13

13

12

10

8

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

原理

Arrestin beta 2

序列

HDHIPLPRPQ S

特异性

This antibody is expected to recognise reported isoforms 1, 2, 3, 5, 6 and 7. No crossreactivity is expected with Arrestin beta 1.

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1.

亚型

IgG
anti-Arrestin, beta 2 (ARRB2) (AA 300-409) antibody
ARRB2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4D2 unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 300-400) antibody
ARRB2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 300-409) antibody
ARRB2 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 3G1 unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 222-369) antibody
ARRB2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 300-410) antibody
ARRB2 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G1 unconjugated

anti-Arrestin, beta 2 (ARRB2) (C-Term) antibody
ARRB2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (Middle Region) antibody
ARRB2 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (Middle Region) antibody
ARRB2 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, 马, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 251-418) antibody
ARRB2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Arrestin, beta 2 (ARRB2) (AA 262-430) antibody
ARRB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: Approx 45 kDa band observed in lysates of cell lines A549 and U251 and in preliminary testing of KNRK cell lysate (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 kDa according to NP_945355.1) Recommended concentration: 0.5-1 μg/mL
Peptide ELISA: antibody detection limit dilution 1:64000.

说明

Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Zakrzewicz, Krasteva, Wilhelm, Dietrich, Wilker, Padberg, Wygrecka, Grau: "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys." in: Immunobiology, Vol. 216, Issue 7, pp. 854-61, (2011) (PubMed).

Napolitano, Pasqualetti, Usiello, Santini, Pacini, Sciamanna, Errico, Tassone, Di Dato, Martella, Cuomo, Fisone, Bernardi, Mandolesi, Mercuri, Standaert, Pisani: "Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia." in: Neurobiology of disease, Vol. 38, Issue 3, pp. 434-45, (2010) (PubMed).
抗原

Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

别名

ARRB2 (ARRB2 产品)

别名

ARRB2 antibody, arb2 antibody, arr2 antibody, arrestin antibody, barr2 antibody, betaarr2 antibody, ARB2 antibody, ARR2 antibody, BARR2 antibody, BARRES antibody, AI326910 antibody, AW122872 antibody, arrb2 antibody, zgc:64007 antibody, arrestin beta 2 antibody, arrestin, beta 2 antibody, arrestin beta 2 L homeolog antibody, arrestin, beta 2b antibody, ARRB2 antibody, arrb2 antibody, Arrb2 antibody, arrb2.L antibody, arrb2b antibody

背景

ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3

分子量

46.1kDa

基因ID

409, 216869, 25388

NCBI登录号

NP_004304, NP_945355, NP_001244257, NP_001244259, NP_001244260

途径

Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Phototransduction, Thromboxane A2 Receptor Signaling