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Acetylcholinesterase 抗体 (C-Term)

Verified AChE 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185357
发货至: 中国
  • 抗原 See all Acetylcholinesterase (AChE) 抗体
    Acetylcholinesterase (AChE)
    抗原表位
    • 15
    • 5
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    • 2
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    • 1
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    • 1
    • 1
    C-Term
    适用
    • 51
    • 32
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    • 2
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    • 1
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    宿主
    • 88
    • 10
    • 7
    • 1
    山羊
    克隆类型
    • 97
    • 9
    多克隆
    标记
    • 55
    • 20
    • 8
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    • 1
    • 1
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    • 1
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    This Acetylcholinesterase antibody is un-conjugated
    应用范围
    • 83
    • 48
    • 21
    • 15
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    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    ACHE
    序列
    QFDHYSKQDR CSDL
    特异性
    This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.
    亚型
    IgG
  • 应用备注

    Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    Acetylcholinesterase (AChE)
    别名
    ACHE (AChE 产品)
    别名
    ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody
    背景
    ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
    基因ID
    43
    NCBI登录号
    NP_000656, NP_001269378
    途径
    Skeletal Muscle Fiber Development
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