PDE5A 抗体 (C-Term)
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- 抗原 See all PDE5A 抗体
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PDE5A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- PDE5A
- 序列
- LINGESGQAK RN
- 特异性
- This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
- 交叉反应
- Cow, 犬, 人, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
- 亚型
- IgG
- Top Product
- Discover our top product PDE5A Primary Antibody
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- 应用备注
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Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
- 别名
- PDE5A (PDE5A 产品)
- 别名
- CGB-PDE antibody, CN5A antibody, PDE5 antibody, PDE5A2 antibody, Cgbpde antibody, Cn5n antibody, Pde5 antibody, Pde5a1 antibody, PDE5A antibody, pde5a antibody, cgb-pde antibody, cn5a antibody, pde5 antibody, pde5a1 antibody, phosphodiesterase 5A antibody, phosphodiesterase 5A, cGMP-specific antibody, phosphodiesterase 5A S homeolog antibody, phosphodiesterase 5A, cGMP-specific, b antibody, PDE5A antibody, Pde5a antibody, pde5a.S antibody, pde5ab antibody, pde5a antibody
- 背景
- PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
- 基因ID
- 8654
- NCBI登录号
- NP_001074, NP_246273, NP_236914
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
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