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PDE5A 抗体 (C-Term)

Verified PDE5A 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185350
发货至: 中国
  • 抗原 See all PDE5A 抗体
    PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
    抗原表位
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    C-Term
    适用
    • 25
    • 9
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 22
    • 3
    山羊
    克隆类型
    • 23
    • 2
    多克隆
    标记
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PDE5A antibody is un-conjugated
    应用范围
    • 18
    • 12
    • 9
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    PDE5A
    序列
    LINGESGQAK RN
    特异性
    This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
    交叉反应
    Cow, 犬, 人, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
    亚型
    IgG
    Top Product
    Discover our top product PDE5A Primary Antibody
  • 应用备注

    Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
    别名
    PDE5A (PDE5A 产品)
    别名
    CGB-PDE antibody, CN5A antibody, PDE5 antibody, PDE5A2 antibody, Cgbpde antibody, Cn5n antibody, Pde5 antibody, Pde5a1 antibody, PDE5A antibody, pde5a antibody, cgb-pde antibody, cn5a antibody, pde5 antibody, pde5a1 antibody, phosphodiesterase 5A antibody, phosphodiesterase 5A, cGMP-specific antibody, phosphodiesterase 5A S homeolog antibody, phosphodiesterase 5A, cGMP-specific, b antibody, PDE5A antibody, Pde5a antibody, pde5a.S antibody, pde5ab antibody, pde5a antibody
    背景
    PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
    基因ID
    8654
    NCBI登录号
    NP_001074, NP_246273, NP_236914
    途径
    Regulation of G-Protein Coupled Receptor Protein Signaling
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