COMT 抗体 (N-Term)
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- 抗原 See all COMT 抗体
- COMT (Catechol-O-Methyltransferase (COMT))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This COMT antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 原理
- COMT (N Terminus)
- 序列
- GDTKEQRILN HVLQC
- 特异性
- This antibody is expected to recognise both reported isoforms S-COMT NP_009294.1 and MB-COMT NP_000745.1, NP_001128633.1, NP_001128634..
- 交叉反应
- 犬, 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence GDTKEQRILNHVLQC, from the N Terminus of the protein sequence according to NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1.
- 亚型
- IgG
- Top Product
- Discover our top product COMT Primary Antibody
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- 应用备注
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Western Blot: Approx 25 kDa band observed in Human Liver and Ovary lysates and in MCF7 cell lysates, and approx. 25 kDa and 30 kDa in U251 cell lysate (calculated MW of 30.0 kDa according to NP_000745.1(MB COMT) and 24.5 kDa according to NP_009294.1 (S-COMT)
Peptide ELISA: antibody detection limit dilution 1:4000.
- 说明
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Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- COMT (Catechol-O-Methyltransferase (COMT))
- 别名
- COMT (COMT 产品)
- 背景
- COMT, catechol-O-methyltransferase, OTTHUMP00000197749, OTTHUMP00000197750
- 基因ID
- 1312
- NCBI登录号
- NP_000745, NP_001128633, NP_001128634, NP_009294
- 途径
- Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome
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