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Retinoid X Receptor alpha 抗体 (AA 14-28)

Verified RXRA 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185295
发货至: 中国
  • 抗原 See all Retinoid X Receptor alpha (RXRA) 抗体
    Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))
    抗原表位
    • 7
    • 7
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 14-28
    适用
    • 57
    • 29
    • 26
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 46
    • 9
    • 2
    山羊
    克隆类型
    • 39
    • 17
    多克隆
    标记
    • 33
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Retinoid X Receptor alpha antibody is un-conjugated
    应用范围
    • 41
    • 26
    • 14
    • 7
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    RXR alpha
    序列
    CQVNSSLTSP TGRGSM
    特异性
    This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1.
    亚型
    IgG
  • 应用备注

    Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))
    别名
    RXRA (RXRA 产品)
    别名
    NR2B1 antibody, 9530071D11Rik antibody, Nr2b1 antibody, RXRalpha1 antibody, RXRalpha antibody, rxra-A antibody, xRXR alpha antibody, xrxra antibody, RXRA antibody, RXR alpha antibody, etID309731.5 antibody, rxr antibody, rxra antibody, rxrg antibody, RXRalpha-B antibody, retinoid X receptor alpha antibody, retinoid X receptor alpha L homeolog antibody, retinoid x receptor, alpha b antibody, retinoid X receptor, alpha a antibody, RXRA antibody, Rxra antibody, rxra.L antibody, CpipJ_CPIJ010249 antibody, rxrab antibody, rxraa antibody
    背景
    RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
    基因ID
    6256
    NCBI登录号
    NP_002948
    途径
    Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C
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