Retinoid X Receptor alpha 抗体 (AA 14-28)
-
- 抗原 See all Retinoid X Receptor alpha (RXRA) 抗体
- Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))
-
抗原表位
- AA 14-28
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This Retinoid X Receptor alpha antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- RXR alpha
- 序列
- CQVNSSLTSP TGRGSM
- 特异性
- This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1.
- 亚型
- IgG
-
-
- 应用备注
-
Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))
- 别名
- RXRA (RXRA 产品)
- 别名
- NR2B1 antibody, 9530071D11Rik antibody, Nr2b1 antibody, RXRalpha1 antibody, RXRalpha antibody, rxra-A antibody, xRXR alpha antibody, xrxra antibody, RXRA antibody, RXR alpha antibody, etID309731.5 antibody, rxr antibody, rxra antibody, rxrg antibody, RXRalpha-B antibody, retinoid X receptor alpha antibody, retinoid X receptor alpha L homeolog antibody, retinoid x receptor, alpha b antibody, retinoid X receptor, alpha a antibody, RXRA antibody, Rxra antibody, rxra.L antibody, CpipJ_CPIJ010249 antibody, rxrab antibody, rxraa antibody
- 背景
- RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
- 基因ID
- 6256
- NCBI登录号
- NP_002948
- 途径
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C
-