EHD2 抗体 (C-Term)
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- 抗原 See all EHD2 抗体
- EHD2 (EH-Domain Containing 2 (EHD2))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This EHD2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- EHD2
- 序列
- CRLVPPSKRR HKGSA
- 特异性
- This antibody is expected to recognise EHD1 protein as well as EHD2.
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. R
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- EHD2 (EH-Domain Containing 2 (EHD2))
- 别名
- EHD2 (EHD2 产品)
- 别名
- PAST2 antibody, BC027084 antibody, C130052H20Rik antibody, fj66d02 antibody, wu:fj66d02 antibody, zgc:77671 antibody, MGEPS antibody, MGC64266 antibody, EHD1 antibody, MGC107790 antibody, EH domain containing 2 antibody, EH-domain containing 2 antibody, EH-domain containing 2b antibody, EH domain containing 2 L homeolog antibody, EHD2 antibody, Ehd2 antibody, ehd2b antibody, ehd2.L antibody, ehd2 antibody
- 背景
- EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
- 分子量
- 61.2kDa
- 基因ID
- 30846
- NCBI登录号
- NP_055416
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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