GAPDH 抗体 (C-Term)
(10 references)-
- 抗原 See all GAPDH 抗体
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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抗原表位
- C-Term
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GAPDH antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- GAPDH (C Terminus) Loading Control
- 序列
- HQVVSSDFNS DT
- 特异性
- GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
- 交叉反应
- 犬, 人, 小鼠, Pig
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
- 亚型
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibodyGAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 36 kDa band observed in lysates of cell line HEK293, approx. 37 kDa in Rat Brain lysates and approx.35 kDa in Human Liver and Tonsil lysates, lysates of cell line HeLa and in preliminary testing of Mouse Spleen lysates (calculated MW of
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A549 and HeLa cells. Recommended concentration: 10μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).
: "Minimal effects of spargel (PGC-1) overexpression in a Drosophila mitochondrial disease model." in: Biology open, Vol. 8, Issue 7, (2019) (PubMed).
: "IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells." in: Scientific reports, Vol. 8, Issue 1, pp. 3217, (2019) (PubMed).
: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).
: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).
: "Glioma cell death induced by irradiation or alkylating agent chemotherapy is independent of the intrinsic ceramide pathway." in: PLoS ONE, Vol. 8, Issue 5, pp. e63527, (2013) (PubMed).
: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).
: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).
: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).
: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).
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: "ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).
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- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 别名
- GAPDH (GAPDH 产品)
- 背景
- GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
- 基因ID
- 2597
- NCBI登录号
- NP_002037
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