BAF53A and BAF53B (C-Term) 抗体
Verified
适用: 人, 小鼠, 大鼠
ELISA, WB, FACS, IF
宿主: 山羊
Polyclonal
unconjugated
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- 抗原
- BAF53A and BAF53B
- 抗原表位
- C-Term
- 适用
- 人, 小鼠, 大鼠
- 宿主
- 山羊
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- BAF53A and BAF53B
- 序列
- YEEGGKQCVE RKCP
- 特异性
- This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1.
- 亚型
- IgG
-
- 应用备注
-
Western Blot: Approx 48 kDa band observed in Rat Skeletal Muscle lysates and approx. 50 kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skeletal Muscle lysate.(predicted MW of 47.4 kDa (Actl6a) acco
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- BAF53A and BAF53B
- 别名
- ACTL6A / ACTL6B
- 背景
- BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 com
- 基因ID
- 51412, 86
- NCBI登录号
- NP_004292, NP_817126, NP_057272
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