SMUG1 抗体 (N-Term)
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- 抗原 See all SMUG1 抗体
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SMUG1 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- SMUG1
- 序列
- PQAFLLGSIH EPA
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Preliminary testing showed a band at approx 19 kDa in lysate of cell line HeLa after 0.1 μg/mL antibody staining (calculated MW of 19.6 kDa according to NP_001230718.1),. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U205 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells." in: Cell biochemistry and function, Vol. 27, Issue 4, pp. 228-37, (2009) (PubMed).
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Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells." in: Cell biochemistry and function, Vol. 27, Issue 4, pp. 228-37, (2009) (PubMed).
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- 抗原
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
- 别名
- SMUG1 (SMUG1 产品)
- 别名
- SMUG1 antibody, xsmug1 antibody, 1200013B09Rik antibody, A930006H09Rik antibody, C85220 antibody, FDG antibody, HMUDG antibody, UNG3 antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 antibody, single-strand selective monofunctional uracil DNA glycosylase antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog antibody, SMUG1 antibody, smug1 antibody, smuG1 antibody, smuG antibody, LOC5577584 antibody, CpipJ_CPIJ002767 antibody, Smug1 antibody, smug1.L antibody
- 背景
- SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3, single-strand-selective monofunctional uracil-DNA glycosylase 1, MGC104370
- 基因ID
- 23583
- NCBI登录号
- NP_055126
- 途径
- DNA Damage Repair
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