SH3BP1 抗体 (C-Term)
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- 抗原 See all SH3BP1 抗体
- SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SH3BP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 原理
- SH3BP1
- 序列
- PQPRPRSLAS ETN
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.
Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).
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SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).
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- 抗原
- SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
- 别名
- SH3BP1 (SH3BP1 产品)
- 别名
- ARHGAP43 antibody, 3BP-1 antibody, SH3BP1 antibody, SH3 domain binding protein 1 antibody, SH3-domain binding protein 1 antibody, SH3 domain-binding protein 1 antibody, SH3BP1 antibody, Sh3bp1 antibody, LOC100398528 antibody
- 背景
- SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
- 基因ID
- 23616
- NCBI登录号
- NP_061830
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