KPNA3 抗体 (C-Term)
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- 抗原 See all KPNA3 抗体
- KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This KPNA3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- KPNA3 / IPOA4
- 序列
- DPTANLQTKE FNF
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-DPTANLQTKEFNF, from the C Terminus of the protein sequence according to NP_002258.2.
- 亚型
- IgG
- Top Product
- Discover our top product KPNA3 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.
Western Blot: Approx. 60 kDa band observed in lysates of cell lines CaCo-2, HEK293 and MCF7 (calculated MW of 57.8 kDa according to NP_002258.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS." in: Nature neuroscience, Vol. 18, Issue 9, pp. 1226-9, (2015) (PubMed).
: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).
: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).
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Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS." in: Nature neuroscience, Vol. 18, Issue 9, pp. 1226-9, (2015) (PubMed).
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- 抗原
- KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))
- 别名
- KPNA3 (KPNA3 产品)
- 别名
- IPOA4 antibody, si:dz79f24.2 antibody, importin antibody, SRP1 antibody, SRP1gamma antibody, SRP4 antibody, hSRP1 antibody, ipoa4 antibody, srp1 antibody, srp1gamma antibody, srp4 antibody, karyopherin (importin) alpha 3 antibody, karyopherin alpha 3 (importin alpha 4) antibody, karyopherin subunit alpha 3 antibody, karyopherin alpha 3 (importin alpha 4) L homeolog antibody, Kpna3 antibody, kpna3 antibody, KPNA3 antibody, kpna3.L antibody
- 背景
- KPNA3, IPOA4, karyopherin alpha 3 (importin alpha 4), RP11-432M24.3, SRP1gamma, SRP4, hSRP1, importin alpha 4, importin alpha 4, importin-alpha-Q2, karyopherin alpha 3, SRP1, importin alpha-3
- 基因ID
- 3839, 16648
- NCBI登录号
- NP_002258
- 途径
- Protein targeting to Nucleus
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